Mycoplasma and Chlamydia testing methods for men

When you go to the hospital, you will find that there are men, women, young and old in the hospital. Everyone has different illnesses, so the departments they need to go to for examination are also different, and the measures they take are also different! So, what are the methods for male mycoplasma examination? The following editor will introduce it to you, hoping to help you better understand it.

With the influence of modern life, the detection rates of non-gonococcal urethritis and gonorrhea have increased year by year, and for non-gonococcal urethritis, mycoplasma and chlamydia are mostly tested. For patients with gonorrhea infection, because the symptoms of gonorrhea are more severe, they often cover up the symptoms of mycoplasma and chlamydia infection, making clinical treatment easy to recur and increasing the difficulty of cure. Therefore, for patients with gonorrhea infection, it is also necessary to do mycoplasma and chlamydia testing.

The method of mycoplasma and chlamydia examination in men is as follows:

1. Blood routine: Peripheral blood leukocyte count is generally normal with eosinophilia.

2. X-ray examination: Chest X-rays of Chlamydia pneumonia are nonspecific, mostly showing unilateral lower lobe infiltration, manifesting as segmental pneumonia, and severe cases showing extensive bilateral pneumonia. Chest X-rays of Chlamydia trachomatis pneumonia show extensive bilateral interstitial and alveolar infiltrations. Overinflation signs are more common, and lobar consolidation is occasionally seen.

3. Direct smear microscopy: Take pharyngeal secretions, sputum, respiratory membranes or other parts of the specimens for smear staining. GZmesa stains the protoplasts into red, protoplasts into dark blue, and inclusion bodies of Chlamydia trachomatis into brown because they contain glycogen. 801 stains the protoplasts into brown.

4. Rapid antigen detection: Monoclonal antibody direct immunofluorescence method is often used to detect Chlamydia in samples. Anti-Chlamydia antibody enzyme-labeled antibody 18G and substrate can also be added to perform colorimetric quantitative detection. These two methods are simple and sensitive.

5. Chlamydia isolation. It is best to use Hela cells or Hep-2 cells to culture Chlamydia pneumoniae. Generally, tracheal or nasopharyngeal aspirates are taken as clinical specimens and inoculated in time. Currently, chlamydia identification is mostly done by culturing Hela cells or Hep-2 cells and then using specific monoclonal fluorescent antibody method (MFA). This technology has high sensitivity and strong specificity. If the specimens can be collected early, a positive result can be obtained within 48 hours.

6. Serological examination uses complement binding test. If the antibody titer of recovery phase serum is 4 times or more than that of acute phase serum, it has diagnostic significance, but it has no early diagnostic significance. Microimmunofluorescence (MrF) is applicable to Chlamydia trachomatis.

7. PCR technology: Ordinary PCR technology has the advantages of being fast, simple and specific for detecting Chlamydia pneumoniae-specific DNA. Its sensitivity is higher than that of cell separation technology, but it is not ideal in detecting throat swab specimens. The use of a kit PCR test can significantly improve its sensitivity.

Because male mycoplasma and chlamydia are more sensitive to urethral epithelial cells, male patients often present with typical urethritis symptoms. Mycoplasma and chlamydia will also invade the prostate tissue close to the urethra. After infection with mycoplasma and chlamydia, if the treatment is not timely or improper, the prostate will gradually become infected and symptoms of lesions will appear, and the consequences are disastrous. Therefore, if mycoplasma and chlamydia are detected, in order to avoid causing a greater disease, the patient should be treated in time.