Toxoplasma test for men

Many women will undergo a toxoplasmosis test before and during pregnancy, especially those who have cats or dogs. Of course, in order to give birth to a healthier baby, men also need to undergo a toxoplasmosis test during the pregnancy preparation period, so as to ensure that both the husband and wife have no problems and can conceive normally. So, how do men do a toxoplasmosis test? The following will introduce this!

1. Pathogen Examination

1. Direct microscopic examination of the patient's blood, bone marrow or cerebrospinal fluid, pleural effusion, sputum, bronchoalveolar lavage fluid, aqueous humor, amniotic fluid, etc. for smear, or biopsy of lymph nodes, muscles, liver, placenta, etc., and Wright or Gibbs staining microscopy can find trophozoites or cysts, but the positive rate is not high. Direct immunofluorescence can also be used to examine Toxoplasma gondii in tissues.

2. Animal inoculation or tissue culture Take the body fluid or tissue suspension to be tested and inoculate it into the abdominal cavity of mice. Infection can occur and pathogens can be found. If the first generation of inoculation is negative, blind passage should be performed 3 times. Or tissue (monkey kidney or pig kidney cell) culture can be performed to isolate and identify Toxoplasma.

3. DNA hybridization technology: For the first time, domestic scholars used 32P-labeled probes containing Toxoplasma-specific DNA sequences to perform molecular hybridization with the patient's peripheral blood cells or tissue DNA, showing specific hybridization bands or spots as positive reactions. Both specificity and sensitivity are high. In addition, polymerase chain reaction has been established in China to diagnose this disease, and compared with probe hybridization, animal vaccination and immunological examination methods, it has the advantages of high specificity, sensitivity and rapidity.

2. Immunological examination

1. The antigens used for antibody detection are mainly soluble antigens of tachyzoites (cytoplasmic antigens) and cell membrane antigens. Antibodies of the former appear earlier (detected by staining test, indirect immunofluorescence test), while antibodies of the latter appear later (detected by indirect hemagglutination test, etc.). Using multiple detection methods at the same time can complement each other and increase the detection rate. Since Toxoplasma can exist in human cells for a long time, it is generally difficult to distinguish between current infection and past infection by antibody detection. It can be judged based on the antibody titer and its kinetic changes.

2. Antigen detection uses immunological methods to detect pathogens (tachyzoites or cysts) in host cells, metabolic or cleavage products (circulating antigens) in serum and body fluids. It is a reliable method for early diagnosis and confirmation. Domestic and foreign scholars have established McAb-ELISA and McAb and polyclonal antibody sandwich ELISA methods to detect circulating antigens in the serum of acute patients, and its sensitivity is to detect 0.4μg/ml of antigens in serum.

3. Intradermal test

The antigen is the peritoneal fluid of infected mice or the embryonic fluid of chickens. It often produces a delayed tuberculin reaction. It can be used for epidemiological surveys. It is not widely used at present.