What are the methods for mycoplasma testing in men?

For male friends, it is very necessary to understand the male mycoplasma examination methods. So what are the male mycoplasma examination methods? Next, this article will introduce the male mycoplasma examination methods for your reference. If you want to know what male mycoplasma examination methods are, please continue reading! Please read the detailed introduction below.

Mycoplasma is the smallest microorganism that lives outside cells. It is a type of prokaryotic cell-type microorganism that lacks a cell wall. Its size is generally between 0.3 and 0.5um. It is highly polymorphic and has various forms such as spherical, rod-shaped, filamentous, and branched. It is different from cells and viruses. It has many types and is widely distributed. It causes considerable harm and has an adverse impact on human health and scientific research. Among the 16 types of mycoplasmas isolated from the human body, 5 are pathogenic to humans, namely Mycoplasma pneumoniae, Ureaplasma urealyticum, Mycoplasma hominis, Mycoplasma genitalium and Ureaplasma fermentum, which contain Ureaplasma urealyticum, Ureaplasma urealyticum and Mycoplasma hominis, which are pathogenic to humans.

What are the methods for mycoplasma testing in men? The methods for mycoplasma testing in men are as follows:

1. Blood routine: Peripheral blood leukocyte count is generally normal with eosinophilia.

2. Serological examination: Using complement binding test, if the antibody titer of convalescent serum is 4 times or more than that of acute serum, it has diagnostic significance but no early diagnostic significance. Microimmunofluorescence method is applicable to Chlamydia trachomatis.

3. Direct smear microscopy: Take pharyngeal secretions, sputum, respiratory membranes or other parts of the specimen for smear and stain with GZmesa. The protozoa are stained red, the protozoa are stained dark blue, and the inclusion bodies of Chlamydia trachomatis are stained brown because they contain glycogen 801.

4. Rapid antigen detection: Monoclonal antibody direct immunofluorescence method is often used to detect Chlamydia in samples. The 2I-IsA method can also be used to add anti-Chlamydia antibody enzyme-labeled antibody 18G and substrate for colorimetric quantitative detection. These two methods are simple and sensitive.

5. PCR technology: Ordinary PCR technology has the advantages of being fast and simple to detect Chlamydia pneumoniae-specific DNA, and its sensitivity is higher than that of cell separation technology. However, it is not ideal in detecting throat swab specimens. The use of nested PCR detection can significantly improve its sensitivity.

6. Chlamydia isolation: Hela cells or Hep-2 cells are best used for the culture of Chlamydia pneumoniae. Generally, tracheal or nasopharyngeal aspirates are taken as clinical specimens for timely inoculation. Currently, the identification of Chlamydia is mostly carried out by the specific monoclonal fluorescent antibody method after Hela cells or Hep-2 cells are cultured. This technology has high sensitivity and strong specificity. If the specimens can be collected early, a positive result can be obtained within 48 hours.

The above is the introduction about the methods of mycoplasma testing for men. I believe that after reading the above introduction, you already know the relevant methods of mycoplasma testing for men. From the above introduction, we can see that there are many methods of mycoplasma testing for men. I hope that through the introduction of this article, it will be helpful to friends who want to know the methods of mycoplasma testing for men.