What are the routine sperm chromosome tests?

Routine sperm chromosome examination. For some men who want to have children as soon as possible, they must pass the routine sperm chromosome examination as soon as possible to ensure the health of their sperm. This will make their sperm healthier and increase the pregnancy rate when their lover becomes pregnant. So many men want to fully understand what routine sperm chromosome examinations are. In order for you to have a comprehensive understanding, take a look at the introduction below.

1. Sperm chromosome examination

Diagnosis. Refers to the direct analysis of sperm chromosomes. It includes sperm capacitation, superovulation of golden hamsters, in vitro fertilization, sperm chromosome preparation and chromosome analysis. First, the golden hamster eggs obtained by superovulation are treated with hyaluronidase to digest the granulocytes around the eggs, and the zona pellucida is digested with trypsin. Then, it is incubated with human sperm that has been capacitation treated in advance for 3 hours. The sperm concentration is 1-2×107/ml.

After the sperm and egg combine, wash the eggs several times with BWW culture medium to remove sperm adhering to the surface that has not entered the egg. Continue to incubate the eggs for 12 hours. Finally, treat the eggs with 1% sodium citrate solution for 10 to 15 minutes. When the eggs are fully expanded under a stereomicroscope, they are aspirated out and placed on a clean glass slide. They are fixed with a 3:1 methanol:glacial acetic acid solution, cooled at room temperature, and then stained with Giamsa solution or various banding treatments are performed.

2. Routine inspection items

1. Semen volume:

It is necessary to check the total amount of semen discharged by male friends in one ejaculation. Under normal circumstances, a normal man can discharge 1.5 ml to 6.0 ml.

2. Sperm count (concentration or density):

It is necessary to check how many sperm are in each milliliter of male semen. Under normal circumstances, the number of sperm in male semen is 20 million/ml.

3. Sperm motility:

It is necessary to check the active sperm in the semen of male friends. In the semen of normal male friends, at least 60% of every 100 sperm should be active sperm.

4. Acidity and alkalinity (PH value):

It is also necessary to check the pH value of sperm. Normally, the pH value of male sperm should be kept between 7.0 and 7.8. If the pH value of sperm is less than 7 or greater than 8, it means that the male's sperm motility has decreased significantly.

5. Color:

Normal male semen is milky white or grayish white. If the semen is yellow-green, it means that the man has genital tract infection.

What are the routine sperm chromosome tests? After understanding the above introduction, many people have fully understood the routine sperm chromosome tests, so after fully understanding, they know what items need to be checked, so they can pass the test as soon as possible to ensure the survival rate of their sperm, so as to ensure that their lover's fertilization rate is higher.